Internalization of Trichosanthin via Different Endocytic Mechanisms

Trichosanthin (TCS) is a plant toxin with ribosome-inactivating activity. TCS can be internalized by the host cells and then attacks the ribosomes resulting in cell death. However, the manner for endocytic uptake of TCS is not well understood. The present work investigates the endocytosis pathway of TCS in human choriocarcinoma cells. The different endocytic mechanisms are interfered by potassium depletion, cholesterol-extraction/addition, or treatments of various drugs. The experiments detect their effects on the TCS-uptake. The results show that a large portion of the TCS can be internalized by clathrin-dependent, as well as by clathrin-independent but cholesterol-dependent endocytosis in human choriocarcinoma cells.

作 者： ZHANG Fan SUI Senfang   作者單位： Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China  刊 名： 清華大學學報（英文版）  EI 英文刊名： TSINGHUA SCIENCE AND TECHNOLOGY  年，卷(期)： 2005 10(4)  分類號： Q2  關鍵詞： trichosanthin   clathrin-dependent endocytosis   cholesterol-dependent endocytosis  

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